Saturday, June 30, 2007
Ibro VLTP cours in Xi'an
the VLTP course lasting ten days is over. I miss it very much. We are left with great memories with our aspiring, earnest and kind lecturers, who are prominent senior researchers in neuroscientific sphere and who brought knowlege, wisdom, inspiration, and enthusiam for neuroscience to us who are just barely armed and are in badly need of those timely help.
Friday, May 18, 2007
right eye is more susceptible to myopia
personal experience reveal that the right eye tend to develop more sever myopia compared with left eye. it is thus tempting to make systematic and thorough investigation so as to testify such hypothesis.
Sunday, April 22, 2007
How I determine the site of intrastriatal injection
First, construct a picture with skull and brain in their right positions.
Then, find the corresponding site of striatum in mouse brain atlas
the colorful site would be the ideal position for injection
Thursday, March 22, 2007
How we know the exact position of the counterpart of the structures in human brain
Subcortical regions, such as the amygdala, the nucleus accumbens, and the mesotelencephalic dopamine system, have been shown in animal models to promote the self-administration of drugs of abuse. Functional imaging studies have shown that exposure to drug-associated cues activates cortical regions such as the anterior cingulate cortex, the orbitofrontal cortex, and the insula, which are the most likely region that are responsible for drug addiction.
A research group from University of Southern California recently found that smokers with brain damage involving the insula, a region implicated in conscious urges, were more likely than smokers with brain damage not involving the insula to undergo a disruption of smoking addiction. (see details in http://www.sciencemag.org/cgi/content/full/315/5811/531)
I thus wonder whether this experiment could possibly be replicated in mouse or rat model of drug addiction.Anyway, without validated animal models, this phenomenon cannot be further investigated or proved to be useful in practice. Do you think it might be the next step that ensues the initial discovery?
As for my part, it would be necessary to make it sure that it is feasible for experimental test.
I have looked for the word insula cortex in the atlas of mouse brain and found the following names and positions:
AI agranular insular cortex 0, 10-14
AID agranular insular cortex, dorsal part 0, 15-27, 114-132
AIP agranular insular cortex, posterior part 0,28-40, 129-132
AIV agranular insular cortex, ventral part 0,15-27, 114-132
DI dysgranular insular cortex 15-40, 120-132
GI granular insular cortex 18-40, 121-132
Obviously, there is a region in mouse brain corresponding to the insular cortex in human brain. But, further reflection begets the question that how we know the alleged part, very tricky and fine regions, are functioning the same as insular cortex in human brain? That is to say, how do we map the mouse brain and designate names that are used in defining structures in human brain to mouse brain? As far as insular cortex is concerned, the case becomes so difficult especially considering its hidding posture inside the cortex while, by contrast, mice have no such a covert part.
Given that there is indeed a brain region in mouse named insular cortex, which is presumably functioning the same as its counterpart in human brain, it seems that we do have a chance to establish the causal relationship between the impaired insular cortex and loss of addiction in certain mouse model of drug abuse. Do you think so?
A research group from University of Southern California recently found that smokers with brain damage involving the insula, a region implicated in conscious urges, were more likely than smokers with brain damage not involving the insula to undergo a disruption of smoking addiction. (see details in http://www.sciencemag.org/cgi/content/full/315/5811/531)
I thus wonder whether this experiment could possibly be replicated in mouse or rat model of drug addiction.Anyway, without validated animal models, this phenomenon cannot be further investigated or proved to be useful in practice. Do you think it might be the next step that ensues the initial discovery?
As for my part, it would be necessary to make it sure that it is feasible for experimental test.
I have looked for the word insula cortex in the atlas of mouse brain and found the following names and positions:
AI agranular insular cortex 0, 10-14
AID agranular insular cortex, dorsal part 0, 15-27, 114-132
AIP agranular insular cortex, posterior part 0,28-40, 129-132
AIV agranular insular cortex, ventral part 0,15-27, 114-132
DI dysgranular insular cortex 15-40, 120-132
GI granular insular cortex 18-40, 121-132
Obviously, there is a region in mouse brain corresponding to the insular cortex in human brain. But, further reflection begets the question that how we know the alleged part, very tricky and fine regions, are functioning the same as insular cortex in human brain? That is to say, how do we map the mouse brain and designate names that are used in defining structures in human brain to mouse brain? As far as insular cortex is concerned, the case becomes so difficult especially considering its hidding posture inside the cortex while, by contrast, mice have no such a covert part.
Given that there is indeed a brain region in mouse named insular cortex, which is presumably functioning the same as its counterpart in human brain, it seems that we do have a chance to establish the causal relationship between the impaired insular cortex and loss of addiction in certain mouse model of drug abuse. Do you think so?
Sunday, March 18, 2007
how to manipulate molecules dissolved in water
it is a significant idea to have drug molecules to align or move in an intended way.
I have several way toward this.
1.magnetic field. is there any possibility to associate molecules with magnetic force so that they could be commanded.
I have several way toward this.
1.magnetic field. is there any possibility to associate molecules with magnetic force so that they could be commanded.
Wednesday, February 28, 2007
Tuesday, February 27, 2007
experimental pictures out of my hands
Fascinated by the neurobiological world, I am much intrigued by the amazing world revealed by modern scientific technologies such as morphological study, molecular biological study or physiological tools, etc.
dopaminergic neurons
Microglia /cd11b
dopaminergic neurons
Microglia /cd11b
A Brief Personal statement
Ever since in high school, inspired by the great deeds of well known scientists like Archimedes, Copernicus, Einstein, and so forth, I have determined to become a scientist and began my pursue for science career. I got brilliant academic record during undergraduate study and obtained my bachelor’s degree in clinical medicine. Afterwards, exempted of examination, I went to pursue my master’s degree in neurobiology for the reason that I am too much intrigued by the amazing power of brain and its complex delicate structures that materialize the mind. It is my area of fascination.
Being a graduate student engaged in neuroscientific research, I earnestly studied the courses related to my majoring and set my hands on experiments. As a cooperation project of department of immunology and my supervisor, I chose the mouse model of PD and investigated the role of complement, a component of innate immune system, in the pathogenesis of MPTP induced neurodegeneration of dopamine neurons in Snpc, harnessing complement 3 knock out mice. It was a challenging task in our lab since no one has ever done this before, involving the making of the injury model and large amount of article reading. Although I failed to obtain a positive result in the main project, a parallel idea did work, which provide the clue for me to proceed and finally composed a paper which is just the English publication in my CV(it is also attached to the letter, if you don’t mind).
Aside from the techniques relevant to the main project, I have learned some other basic experimental techniques, such as Western blot, ELISA and some molecular engineering processes. Admittedly, I can not say I have mastered these techniques. I just know something about them.
Through my diligence and intelligence, I obtained my master’s degree with flying color since I am the only graduate who has published a paper in SCI English journals during 3 years of master’s study out of more than 200 graduates. Although Neuroscience letter is not an eminent journal and my work is not worth much of mention, I still feel grateful to the editor and to God since it is not an easy job to make it under such unfavorable circumstance, plus I wrote, submitted and defended it all by myself.
As an aspring young man, I am still looking for chances of going out to better improve myself since the past three years of graduate study makes me aware of the necessity of going to first class of labs if I want to make my mark in life. I am sure that I can still grow, whereas another three years research in here may mercilessly snuff out my academic life. I yearn for a fruitful life.
2006-9-4
Being a graduate student engaged in neuroscientific research, I earnestly studied the courses related to my majoring and set my hands on experiments. As a cooperation project of department of immunology and my supervisor, I chose the mouse model of PD and investigated the role of complement, a component of innate immune system, in the pathogenesis of MPTP induced neurodegeneration of dopamine neurons in Snpc, harnessing complement 3 knock out mice. It was a challenging task in our lab since no one has ever done this before, involving the making of the injury model and large amount of article reading. Although I failed to obtain a positive result in the main project, a parallel idea did work, which provide the clue for me to proceed and finally composed a paper which is just the English publication in my CV(it is also attached to the letter, if you don’t mind).
Aside from the techniques relevant to the main project, I have learned some other basic experimental techniques, such as Western blot, ELISA and some molecular engineering processes. Admittedly, I can not say I have mastered these techniques. I just know something about them.
Through my diligence and intelligence, I obtained my master’s degree with flying color since I am the only graduate who has published a paper in SCI English journals during 3 years of master’s study out of more than 200 graduates. Although Neuroscience letter is not an eminent journal and my work is not worth much of mention, I still feel grateful to the editor and to God since it is not an easy job to make it under such unfavorable circumstance, plus I wrote, submitted and defended it all by myself.
As an aspring young man, I am still looking for chances of going out to better improve myself since the past three years of graduate study makes me aware of the necessity of going to first class of labs if I want to make my mark in life. I am sure that I can still grow, whereas another three years research in here may mercilessly snuff out my academic life. I yearn for a fruitful life.
2006-9-4
A Letter to former president of IBRO
Dear Prof Lund, August, 2006
How have you been lately? I am Mike from third military medical college, chongqing. Do you remember me? Hope you had a wonderful time in chongqing.
You know, I just got my master’s degree this june and am supposed to complete my phD study in the following 3 years in here. No matter how well my things are going on, I can’t stop worrying about my future, because the 3 years of scientific experience in chongqing has led me to draw a conclusion: this is not a good place for the growth of young scientist.
I remembered professor Cai ever told you about the problems we confront. But I don’t think she got the point. Less developed areas, like chongqing, do not lack fund that badly; what they lack is scientific culture.
Because of historical reasons, science has never got great financial or political support from government until recent years. Therefore, the primary accumulation in science (I mean free studies in natural science) just began in the past 20 years. For developed areas, the large investment brings well equipped working environments and attracts brains, which is the reason why there are now several world-class labs in Shanghai and Beijing in China. Brains and pride make the birth of scientific culture a reality.
For less developed areas, the initiating time point lags at least 10 years behind. Lack of fund leads to lack of well equipped labs and consequently lack of talents. Compared with eastern areas, western areas in china have been trapped by a vicious circle that is broadening the gap between east and west in china and alienating less developed areas in academic research. As a result, the accumulation of scientific spirit or technological training in developing areas is always dangling at the bottom of academic ranking list.
For most graduate students involved in scientific studies, they will be greatly influenced by the initial working environment and mentor. If you grow up (scientifically) from the soil without culture, you might die in scientific career. I have seen too many examples who tried to step in science with passion and ended up with desperation for lack of nutrients. It is hard to imagine a green horn can become a qualified researcher under the leadership of a scientifically apathetic supervisor. To make it worse, the supervisor may never get any training in scientific thinking, no SCI publication either.
I want to become a scientist and try my best to fight the wind that blows me awry. I got brilliant academic record during undergraduate study and obtained my bachelor’s degree in clinical medicine. Afterwards, I was exempted of examination and went to pursue my master’s degree in neurobiology. I choose neurobiology as my major because I am too much intrigued by the amazing power of brain and its complex delicate structures that materialize the mind. My hard work was repaid by the publication of an SCI journal. Although it is not an important journal and my work is not worth much of mention, I still feel grateful to the editor and to God since it is not an easy job to make it under such unfavorable circumstance.
Three years of graduate study makes me more assure of my decision to find better environment. I can still grow. I am afraid another three years of phD study in here will definitely snuff out my academic life. I can’t get better technique training or inspiring exchanges of mind. I yearn for a fruitful life.
So, I don’t know whether it is convenient for you to give me any help. I want to get good trainings in world class labs and work together with young people full of ideas and enthusiasm, people of my kind. Can you give some advice?
All the best
How have you been lately? I am Mike from third military medical college, chongqing. Do you remember me? Hope you had a wonderful time in chongqing.
You know, I just got my master’s degree this june and am supposed to complete my phD study in the following 3 years in here. No matter how well my things are going on, I can’t stop worrying about my future, because the 3 years of scientific experience in chongqing has led me to draw a conclusion: this is not a good place for the growth of young scientist.
I remembered professor Cai ever told you about the problems we confront. But I don’t think she got the point. Less developed areas, like chongqing, do not lack fund that badly; what they lack is scientific culture.
Because of historical reasons, science has never got great financial or political support from government until recent years. Therefore, the primary accumulation in science (I mean free studies in natural science) just began in the past 20 years. For developed areas, the large investment brings well equipped working environments and attracts brains, which is the reason why there are now several world-class labs in Shanghai and Beijing in China. Brains and pride make the birth of scientific culture a reality.
For less developed areas, the initiating time point lags at least 10 years behind. Lack of fund leads to lack of well equipped labs and consequently lack of talents. Compared with eastern areas, western areas in china have been trapped by a vicious circle that is broadening the gap between east and west in china and alienating less developed areas in academic research. As a result, the accumulation of scientific spirit or technological training in developing areas is always dangling at the bottom of academic ranking list.
For most graduate students involved in scientific studies, they will be greatly influenced by the initial working environment and mentor. If you grow up (scientifically) from the soil without culture, you might die in scientific career. I have seen too many examples who tried to step in science with passion and ended up with desperation for lack of nutrients. It is hard to imagine a green horn can become a qualified researcher under the leadership of a scientifically apathetic supervisor. To make it worse, the supervisor may never get any training in scientific thinking, no SCI publication either.
I want to become a scientist and try my best to fight the wind that blows me awry. I got brilliant academic record during undergraduate study and obtained my bachelor’s degree in clinical medicine. Afterwards, I was exempted of examination and went to pursue my master’s degree in neurobiology. I choose neurobiology as my major because I am too much intrigued by the amazing power of brain and its complex delicate structures that materialize the mind. My hard work was repaid by the publication of an SCI journal. Although it is not an important journal and my work is not worth much of mention, I still feel grateful to the editor and to God since it is not an easy job to make it under such unfavorable circumstance.
Three years of graduate study makes me more assure of my decision to find better environment. I can still grow. I am afraid another three years of phD study in here will definitely snuff out my academic life. I can’t get better technique training or inspiring exchanges of mind. I yearn for a fruitful life.
So, I don’t know whether it is convenient for you to give me any help. I want to get good trainings in world class labs and work together with young people full of ideas and enthusiasm, people of my kind. Can you give some advice?
All the best
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